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Table 1 Osteoblast related marker primers used for RT-PCR

From: PINK1 deficiency impairs osteoblast differentiation through aberrant mitochondrial homeostasis

Gene name Primer sequences
(5'–3')
Annealing temperature (℃) Product size (bp)
ALP Forward: CCC ACG TTT TCA CAT TCG GT 57 190
Reverse: GCC TGG TAG TTG TTG TGA GC
BSP Forward: TTT ATC CTC CTC TGA AAC GGT 55 110
Reverse: GTT TGA AGT CTC CTC TTC CTC C
OCN Forward: GCG CTC TGT CTC TCT GAC CT 58 225
Reverse: TTT GTA GGC GGT CTT CAA GC
OPN Forward: ACA CTT TCA CTC CAA TCG TCC 58 240
Reverse: TGC CCT TTC CGT TGT TGT CC
PINK1 Forward: CAC ACT GTT CCT CGT TAT GAA GA 56 157
Reverse: CTT GAG ATC CCG ATG GGC AAT
18S Forward: CTG AGA AAC GGC TAC CAC ATC 58 107
Reverse: GCC TCG AAA GAG TCC TGT ATT G
  1. ALP alkaline phosphatase, BSP bone sialoprotein, OCN osteocalcin; OPN osteopontin, PINK1 PTEN-induced kinase 1