Table 1 Osteoblast related marker primers used for RT-PCR
From: PINK1 deficiency impairs osteoblast differentiation through aberrant mitochondrial homeostasis
Gene name | Primer sequences (5'–3') | Annealing temperature (℃) | Product size (bp) |
|---|---|---|---|
ALP | Forward: CCC ACG TTT TCA CAT TCG GT | 57 | 190 |
Reverse: GCC TGG TAG TTG TTG TGA GC | |||
BSP | Forward: TTT ATC CTC CTC TGA AAC GGT | 55 | 110 |
Reverse: GTT TGA AGT CTC CTC TTC CTC C | |||
OCN | Forward: GCG CTC TGT CTC TCT GAC CT | 58 | 225 |
Reverse: TTT GTA GGC GGT CTT CAA GC | |||
OPN | Forward: ACA CTT TCA CTC CAA TCG TCC | 58 | 240 |
Reverse: TGC CCT TTC CGT TGT TGT CC | |||
PINK1 | Forward: CAC ACT GTT CCT CGT TAT GAA GA | 56 | 157 |
Reverse: CTT GAG ATC CCG ATG GGC AAT | |||
18S | Forward: CTG AGA AAC GGC TAC CAC ATC | 58 | 107 |
Reverse: GCC TCG AAA GAG TCC TGT ATT G |