Fig. 6
From: Recent strategies for enhancing the therapeutic efficacy of stem cells in wound healing
Reproduced from the article by authors Jun et al. [22], copyright 2014, Jun et al.
The effects of hypoxia on MSCs and the effects of their hypoxic conditioned media on fibroblasts and wound closure [22]. A The growth of MSCs and the expression of HIF1-α protein under normoxic or hypoxic conditions (1% or 5% O2); B the expression levels of TGF-β1 and VEGF were confirmed in MSCs and their conditioned media; C the clonogenic capacity of MSCs was detected by CFU-F assay; D BrdU assay was performed to determine the growth of fibroblasts in different conditioned media; E scratch-wound-closure assays were performed to confirm the migration of fibroblasts at the wound site; arrows show the distance of wound; F the images of wounds size; G histomorphometric analysis of wound closure. HIF-1α: inducible transcription factor 1α; nor-CM: normoxic conditioned media; hypo-CM: hypoxic conditioned media; con-CM: conditioned media; CFU-F: colony-forming unit fibroblast; TGF-β: transforming growth factor-β; VEGF: vascular endothelial growth factor.