Fig. 5

Histone demethylase JHDM1D is associated with SATB2-mediated osteo/odontogenic differentiation of hDPSCs. a, b The expression of histone demethylase JHDM1D was increased after SATB2 knockdown, as assessed by quantitative RT-PCR and western blot analysis. c Alizarin Red S staining showed that JHDM1D knockdown upregulated mineralized nodule formation capacity of hDPSCs. d Quantitative RT-PCR showed that DKK1 expression was down-regulated after JHDM1D knockdown. e Western blot analysis showed that JHDM1D knockdown upregulated the level of H3K9me2 and H3K27me2, and increased the expression of active β-catenin. Scale bar 200 μm, *P < 0.05, **P < 0.01, ***P < 0.001. Data are expressed as the means + SD. Each experiment was repeated three times with n ≥ 3 samples per group. si NC: negative control siRNA transfection, si SATB2: SATB2 siRNA transfection, si JHDM1D: JHDM1D siRNA transfection