Fig. 2

BMSC transplantation rescues mitochondrial dysfunction. A TEM micrographs of isolated mHCs from ND mice and T2D-associated NAFLD mice administered with vehicle or BMSCs at 45th week. B The average number of mitochondria in each cell. The statistics are from random 25 cell fields per mice and 6 mice per group. C OCR analysis of isolated mHCs from ND mice and T2D-associated NAFLD mice administered with vehicle or BMSCs at 45th week. The mHCs come from 6 mice per group. D The ATP detection of isolated mHCs. The mHCs were isolated from 6 mice per group. E Real-time PCR describes the mtDNA/nDNA ration according to the previous report [60]. Relative quantification was performed on 6 animals per group. F The mRNA expression level of mitochondria-related genes in terms of dynamics and biogenesis. The RNA was extracted from homogenized liver tissues of 6 mice per group. G MDA detection of homogenized liver tissues of 6 mice per group. H, I MMP and ROS detection of mHCs from 6 mice per group. All statistical data are represented as means ± s. *P < 0.05; **P < 0.01; ***P < 0.001