Table 1 Primer pairs sequences against osteoclast gene used in qPCR
From: DZNep promotes mouse bone defect healing via enhancing both osteogenesis and osteoclastogenesis
Mouse gene | Forward 5′ → 3′ | Reverse 5′ → 3′ |
|---|---|---|
Trap/Acp5 | CAAAGAGATCGCCAGAACCG | GAGACGTTGCCAAGGTGATC |
Ctsk | CTTCCAATACGTGCAGCAGA | TCTTCAGGGCTTTCTCGTTC |
Atp6v0d2 | GCAGAGCTGTACTTCAATGTGG | TAGTCCGTGGTCTGGAGATG |
Calcr | TCTGCGTTCCTGAGAACACC | AAGGCGCTCTAATGGCACTT |
Nfatc1 | TGCTCCTCCTCCTGCTGCTC | GCAGAAGGTGGAGGTGCAGC |
c-Fos | CCAGTCAAGAGCATCAGCAA | AAGTAGTGCAGCCCGGAGTA |
Runx2 | TGGCCGGGAATGATGAGAAC | GGATGAGGAATGCGCCCTAA |
Alpl | GGGCATTGTGACTACCACTCG | CCTCTGGTGGCATCTCGTTAT |
Ocn | GCGCTCTGTCTCTCTGACCT | TTTGTAGGCGGTCTTCAAGC |
Opn | TTTGTAGGCGGTCTTCAAGC | GTGAGATTCGTCAGATTCATCCG |
Col1a1 | TAAGGGTCCCCAATGGTGAGA | GGGTCCCTCGACTCCTACAT |
Wnt1 | ATAGCCTCCTCCACGAACCT | GATGAACGCTGTTTCTCGGC |
Wnt4 | GAGCAACTGGCTGTACCTGG | GGAACTGGTACTGGCACTCC |
Wnt6 | GGTCACTCAAGCCTGTTCCA | CCGAAGTCCACATCGTCTCC |
Wnt10a | CTGAACACCCGGCCATACTTC | CTGAACACCCGGCCATACTTC |
Foxc1 | CACTCGGTGCGGGAAATGT | GTGCGGTACAGAGACTGACTG |
Actb | ACAGCAGTTGGTTGGAGCAA | ACGCGACCATCCTCCTCTTA |