Fig. 4

The effects of Muse cell supernatant on wound healing and the proliferation of keratinocytes. A Muse cell suspensions were inoculated into the plug-in chamber, and then the cells were cultured for 1 day. Cell migration was observed, and photos were taken at 0 h, 6 h and 12 h in the different groups. The area at 0 h was used as a control. The results showed that compared with that in the control group, the migration rate of HaCaT cells in the Muse cell supernatant treatment group was significantly accelerated at 6 and 12 h. B HaCaT cells were cultured on glass coverslips, and then Muse cell supernatant was added and incubated for 30 min. Cell proliferation was observed, and photos were obtained after immunofluorescence staining. The proliferation of HaCaT cells was slightly accelerated in the Muse cell supernatant treatment group compared with the vehicle group. C Statistical analysis of HaCaT cell migration areas. Two-way ANOVA followed by the Bonferroni test; ***p < 0.001versus 0 h; n = 9 slices in each group. The data are presented as the mean ± s.e.m. D Statistical analysis of EdU analysis of HaCaT cell proliferation. Two-tailed Student’s t-test; *p < 0.05 versus vehicle group; n = 15 slices from 5 cultures per group. The data are presented as the mean ± s.e.m