Table 1 Antibodies used in characterization of limbal stem cell marker expression
Antibody | Host | Dilution | Manufacturer | Catalog #No |
|---|---|---|---|---|
PAX6 | Rabbit | 1:100 | Sigma-Aldrich | # HPA030775 |
ABCG2 | Mouse | 1:200 | Merck Millipore | # MAB4155 |
ABCB5 | Mouse | 1:200 | Frank Lab* | - |
p63α | Rabbit | 1:200 | Cell Signaling Technology | # 4892 |
p40 (∆Np63) | Mouse | 1:100 | BioCare Medical | # ACI3066 |
CK3 | Mouse | 1:100 | Invitrogen | # MA1-5763 |
CK12 | Rabbit | 1:200 | Abcam | # AB185627 |
CK14 | Mouse | 1:200 | R&D Systems | # MAB3164 |
CK15 | Mouse | 1:200 | ThermoFisher Scientific | # MS-1068-P1 |
Anti-rabbit IgG, Alexa Fluor 488 | Donkey | 1:800 | Molecular Probes | # A21206 |
Anti-mouse IgG, Alexa Fluor 568 | Donkey | 1:800 | Molecular Probes | # A10037 |
Anti-goat IgG, Alexa Fluor 647 | Donkey | 1:800 | Abcam | # AB150131 |