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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Functional tissue-engineered microtissue formed by self-aggregation of cells for peripheral nerve regeneration

Fig. 3

Indirectly co-culture each group and GFP-SCs or DRGs by a transwell system. a The up row is the indirect co-culture of each group with GFP-SCs. GFP-SCs exhibited green fluorescence. The bottom row is the indirect co-culture of each group with DRGs. The axons of DRG exhibited green fluorescence, SCs that migrated from the DRG exhibited red fluorescence, and all nuclei showed blue fluorescence. Scale bars: 100 μm (up row) and 500 μm (bottom row). b The number of SCs (per ×10 magnification field) in the Microtissue group was significantly higher than the 2D group (p < 0.0001) and the Control group (p < 0.0001). c The axon length of DRG in the Microtissue group was significantly longer than that in the 2D group (p < 0.001) and the Control group (p < 0.0001)

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