Fig. 3

GDM-like milieu confers a pro-angiogenic profile on NGT-hAMSCs. NGT-hAMSCs were cultured in the presence of 30 mM glucose, 100 nM insulin and 50 µM palmitic acid. A Visual representation of tube formation assay of NGT-hAMSCs and GDM-like treated hAMSCs. Two representative fields of each condition are shown. Scale bars = 100 µm B Differences in the number of extremities, nodes, junctions and meshes between groups (N = 10 each group). C Protein expression analysis comparing angiogenic protein levels of FGFR2, TGFBR1, PAI-1 and VEGFA between groups. Representative image and densitometry analysis (arbitrary units, N = 9 each group). Data are normalized to the mean of the controls of each experiment and are shown as mean ± SEM. Differences assessed by Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001