Fig. 1

Proliferation, growth rate kinetic, doubling time comparison. A. Representative images of HCaMSC (a) and IPFaMSC (b) growth for 2 days in IncuCyte S3 Live Cell Analysis instrument. All tested cells lines exhibit spindle-shaped adherent morphology. Nuclei are stained red. Scale bar = 400 μm. B. HCaMSC and IPFaMSC cell proliferation estimated by counting the number of red nuclei for 3 days. C. Unpaired Student's t test analysis of population doubling times of both tested groups. Dots represent individual cell line values. Median values for IPFaMSC and HCaMSC were 17.0 and 16.6 h, respectively. Differences were considered significant when *p < 0.05, compared with control cell lines values. D. Flow cytometry results of IPFaMSCs HCaMSCs phenotypes. All ten cell lines tested more than 95% positive for typical MSC markers CD90, CD73, CD105, CD44 and HLA-ABC expression, and negative for lineage markers CD14, CD45 and HLA-DR expression