Fig. 1

Characterizing the pharmacological effect of UM171a on MSCs. A Assessment of various UM171a doses on the proliferation of MSCs over a 72 h period. For this panel, n = 3/group. B Phenotypic analysis of MSCs treated with 1000 nM UM171a for 72 h. The dashed lines represent isotype signals. The isotype controls for DMSO and UM171a are shown in light gray and red, respectively. The isotype and marker staining for DMSO are shown in light and dark gray, respectively. The isotype and msrker staining for UM171a are shown in light and dark red, respectively. C Timeline comparison of the effect of three UM171a doses on H2-K^b induction. D Testing the effect of UM171a on H2-K^b using doses higher than 1000 nM. E Flow analysis of EPCR cell surface expression on MSCs treated with 1000 nM UM171a for 72 h. F Representative flow-cytometry analysis of HLA-A/B/C on the surface of human UC-derived MSCs treated with UM171a at 35, 250 and 1000 nM. All experiments were repeated at least three times