Fig. 2

ADSCs transfected with IGF-1 modRNA promoted extracellular matrix synthesis of chondrocytes. A Schematic model of indirect co-culture system of chondrocytes with ADSCs, GFP-ADSCs or IGF-1-ADSCs using transwell in the presence of IL-1β (chondrocytes without co-culture, the control group). Each cell type was grown independently on the transwell plates. B, C Representative photo-micrographs and fluorescent intensity analysis of immunofluorescence staining for aggrecan and type II collagen in mouse primary chondrocytes cocultured with ADSCs, GFP-ADSCs or IGF-1-ADSCs for 48 h (Scale bar = 100 μm). D Quantitative polymerase chain reaction analysis of mRNA transcript levels of Col2a1 and Acan in mouse primary chondrocytes cocultured with ADSCs, GFP-ADSCs, IGF-1-ADSCs for 48 h (chondrocytes without co-culture, the control group). (*P < 0.05, **P < 0.01, ***P < 0.001, ns not significant)), COL2 type II collagen, IL-1β interleukin 1 beta