Fig. 3

Extracellular vesicle (EV) profiling within media conditioned by cell products. A Proteomic antibody array showed the presence of 8 known EV markers (ICAM, ALIX, CD81, CD63, EPCAM, ANXAS, TSG101, FLOT-1) and the absence of bands for cis-Golgi marker (GM130) suggesting that EV preparations were free of cellular contaminants. B Nanoparticle tracking analysis showed the concentration and size of EVs isolated from all 3 cell types were representative of accepted definitions for EV identity and no significant differences were found between cell products. All data are presented as individual and mean values ± SEM, n = 3 biological replicates; each circle represents one data point from one unique biological replicate. Differences between cell types were analyzed by One-way ANOVA