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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: The cannabinoid receptor I (CB1) enhanced the osteogenic differentiation of BMSCs by rescue impaired mitochondrial metabolism function under inflammatory condition

Fig. 1

CB1 promoted the osteogenic differentiation of hBMSCs. A Western blot results showed the knockdown efficiency of CB1 shRNA1 in BMSCs. β-actin was used as an internal control. B The ALP activity assay in CB1 depleted hBMSCs. C Alizarin red staining in CB1 depleted hBMSCs. D OD values of the alizarin red staining in CB1 depleted hBMSCs. E Calcium quantitative analysis in CB1 depleted hBMSCs. F Western blot results showed the over-expression efficiency of HA-CB1 in BMSCs. β-actin was used as an internal control. G The ALP activity assay in CB1 over-expressing hBMSCs. H Alizarin red staining in CB1 over-expressing hBMSCs. I OD values of the alizarin red staining in CB1 over-expressing hBMSCs. J Calcium quantitative analysis in CB1 over-expressing hBMSCs. Student’s t test was performed to determine statistical significance. Error bars represent SD (n = 3). *P ≤ 0.05; **P ≤ 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512