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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: The cannabinoid receptor I (CB1) enhanced the osteogenic differentiation of BMSCs by rescue impaired mitochondrial metabolism function under inflammatory condition

Fig. 3

CB1 promoted the osteogenic differentiation of hBMSCs under TNF-α and INF-γ stimulation. A–D 10 ng/ml TNF-α was used to treat BMSCs. A Real-time RT-PCR results showed the expression of CB1 at 1, 2, 4 and 8 h after 10 ng/ml TNF-α treatment in BMSCs. B ALP activity assay. C Alizarin red staining. D Calcium quantitative analysis. E–H 100 ng/ml INF-γ was used to treat BMSCs. E Real-time RT-PCR results showed the expression of CB1 at 1, 2, 4 and 8 h after 100 ng/ml INF-γ treatment in BMSCs. F ALP activity assay. G Alizarin red staining. H Calcium quantitative analysis. GAPDH was used as an internal control. One-way ANOVA was performed to determine statistical significance. Error bars represent the SD (n = 3). *P ≤ 0.05; **P ≤ 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512