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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: The cannabinoid receptor I (CB1) enhanced the osteogenic differentiation of BMSCs by rescue impaired mitochondrial metabolism function under inflammatory condition

Fig. 6

The function of ROT and CoQ10 on the osteogenic differentiation in hBMSCs. A–F 50 nM ROT was used to treat BMSCs. A The ALP activity results. B Alizarin red staining. C Calcium quantitative analysis. D The ALP activity results. E Alizarin red staining. F Calcium quantitative analysis. G 50 nM ROT and 10 ng/ml TNF-α were used to treat BMSCs. The ALP activity results. H 50 nM ROT and 100 ng/ml INF-γ were used to treat BMSCs. The ALP activity results. I–K 30 μM CoQ10 was used to treat CB1sh1 BMSCs. I The ALP activity results. J Alizarin red staining. K Calcium quantitative analysis. L–N 30 μM CoQ10 and 10 ng/ml TNF-α were used to treat CB1sh1 BMSCs. L The ALP activity results. M Alizarin red staining. N Calcium quantitative analysis. O–Q 30 μM CoQ10 and 100 ng/ml INF-γ were used to treat CB1sh1 BMSCs. O The ALP activity results. P Alizarin red staining. Q Calcium quantitative analysis. The One-way ANOVA was performed to determine statistical significance. Error bars represent SD (n = 3). *P ≤ 0.05; **P ≤ 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512