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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: The cannabinoid receptor I (CB1) enhanced the osteogenic differentiation of BMSCs by rescue impaired mitochondrial metabolism function under inflammatory condition

Fig. 7

The effect of CB1 on MAPK signal pathways in hBMSCs. A–C 10 μM AM251 was used to treat the CB1 over-expressed BMSCs for 4 h, 20 μM SB203580 or 20 μM SP600125 was used to treat the CB1 over-expressed BMSCs for 2 h, Western blot results showed the expression of phosphorylated p38 MAPK, JNK and Erk1/2, and p38 MAPK, JNK, and Erk1/2 in BMSCs. Histone H3 was used as an internal control. B, C The Real-time RT-PCR results of Nrf1 (B) and Nrf2 (C) expressions in BMSCs. GAPDH was used as an internal control. One-way ANOVA was performed to determine statistical significance. Error bars represent the SD (n = 3). *P ≤ 0.05; **P ≤ 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512