Fig. 2

Sin3a contributes to the initiation process of reprogramming. A A schematic illustration of the experimental strategy is presented. MEFs were reprogrammed with OSKM retroviruses and infected with a control lentivirus (shCtrl) or a shRNA lentivirus (shSin3a-1) for one day from Day 0 to Day 8. Reprogramming efficiency was measured by counting the number of AP⁺ colonies or GFP⁺ colonies at Day 12. B Representative images of AP staining at Day 12 of reprogramming are shown. C and D The number of AP⁺ colonies (C) or GFP⁺ colonies (D) generated from MEFs infected with control and shSin3a-1 viruses was counted at Day 12. Significance was estimated by student’s unpaired t test. *P < 0.05, **P < 0.01, and ***P < 0.001 versus the control group. Error bars represent SD (n = 3 independent experiments)