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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Disruption of hematopoiesis attenuates the osteogenic differentiation capacity of bone marrow stromal cells

Fig. 4

The differentiation of Pten+/flox and Cre+Pten+/flox MSCs after cross co-culture with different bone marrow blood cells from 5-week-old mice. Aa–f von Kossa staining showing the alteration of extracellular mineral deposition of MSCs after incubation with different bone marrow blood cells. Ag–l Oil Red O staining showing adipocyte formation after incubation with bone marrow blood cells for 3 days and adipo-induction for 10 days. B Quantity of calcium deposition of MSCs in osteo-induction medium after incubation with bone marrow blood cells for 3 days. C The relative mRNA expression of genes related to osteogenesis in Pten+/flox and Cre+Pten+/flox bone marrow cells. Da–f The osteoblastic differentiation and Dg–l adipo-differentiation of Pten+/flox and Cre+Pten+/flox MSCs when Transwells were inserted into co-culture to block direct contact between blood cells and MSCs. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512