Fig. 5

Macrophage depletion reverses the angiogenesis effect of BM-MSCs in fat grafting. a 7 days and 14 days after PBS or BM-MSCs treatments, MAC-2 (red) and CD31 (green) were used to stain control or clodronate liposome-treated adipose tissue sections to label endothelial cells and macrophages, respectively. Scale bar 50 um. b After clodronate liposome treatment, MAC-2 dropped sharply in the PBS and BM-MSCs groups. c Compared with the macrophage depletion group, the blood vessels in the transplanted fat were significantly reduced. n = 6 per group. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001