Fig. 3

Grk3-/- BmMSCs have higher levels of CXCL12 detected in culture and proliferate more in comparison with WT BmMSCs but secrete similar amounts of CXCL12 per cell. (A) Quantification of CXCL12 protein concentration from supernatant of BmMSC cultures. Lower limit of detection at 46.9 ρg/mL. Data represent mean ± SEM, n = 4, passages 11 and 12. (B) BmMSC proliferation determined by increased formazan dye production from viable cells over time using CCK-8 proliferation assay. Data represent mean ± SEM, n = 3, except day 0 n = 2, passage 7. * P ≤ 0.05 ** P ≤ 0.01 *** P ≤ 0.001, **** P ≤ 0.0001 (C) CXCL12 per MSC determined by trypsinized cell count using trypan blue exclusion of individual wells divided by the amount of CXCL12 (pg/ml) measured by ELISA. Data represent mean ± SEM, n = 11 Grk3-/-, n = 10 WT, passages 10–13