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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: G protein-coupled receptor kinase 3 modulates mesenchymal stem cell proliferation and differentiation through sphingosine-1-phosphate receptor regulation

Fig. 6

Grk3-/- BmMSCs have enhanced ERK1/2 signaling after S1P stimulation at later timepoints. (A) WT (left) and Grk3-/- (right) BmMSCs were stimulated with 1 µM S1P for designated times for analysis of ERK1/2 signaling; phospho-ERK (pERK) and total ERK (tERK). Representative blot, n = 4. The pERK/tERK ratio for each blot was quantified via densitometry for (B) early-stage ERK signaling at 0, 1, and 5 min and (C) late-stage ERK signaling at 15, 30, and 60 min. Data represent mean ± SEM, n = 4, passages 13 and 15. ** P ≤ 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512