Fig. 1

Identification of stem cell quality and HLA-I expression of iPSC lines. A Morphology observation of different PSC lines with phase contrast microscope: iPSC lines (C10, C55, C56 and UHI-F MI) and ESC lines (H1, H9 and HN4) were grown in the identical BioCISO medium to compare their undifferentiated morphology, while primary hUCs with epithelioid morphology were used as a differentiated control. Scale bars, 100 µm. B Relative expression level of marker genes specific for the three germ layers on the EB differentiation of C55 line by qRT-PCR. The original C55 cells served as undifferentiated control. Relative quantification was normalized to GAPDH (n = 3 replicates). Statistical significance was determined with two-way ANOVA followed by Tukey’s multiple comparison test. Data are means ± SD; ***p < 0.001. C G-banding analysis of C55 line showed normal karyotype. D FCM analysis of HLA-I expression on the different PSCs. Cells were stained with anti-HLA-ABC-PE (solid line) and isotype control (dotted line) antibodies. MFI of HLA-ABC staining is shown inside the figures