Fig. 3From: Therapeutic effect of adipose stromal vascular fraction spheroids for partial bladder outlet obstruction induced underactive bladderAd-SVF spheroids promoted angiogenesis. A Representative images of tube formation assay under different culture medium. B–E Quantification of total length, total segment length, number of branches, and number of nodes were analyzed by ImageJ’s angiogenesis plugin. F Representative images of CM-DiI labelled cells (red), the expression of CD31 by immunofluorescence (green), and nuclear marked DAPI (blue) in Matrigel of each group (Arrows represent vessel). G, H Semi-quantitative analysis of the mean percentage of CD31 positive cells and the mean fluorescent intensity of CD31. Scale bar = 100 μm. *p < 0.05 (ad-SVF, ad-SVFsp vs Sham); #p < 0.05 (ad-SVFsp vs ad-SVF)Back to article page