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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: CRISPR single base editing, neuronal disease modelling and functional genomics for genetic variant analysis: pipeline validation using Kleefstra syndrome EHMT1 haploinsufficiency

Fig. 2

EHMT1_WT and EHMT1_SNV neural cell differentiation. Cells were subject to neural progenitor cell differentiation with cells harvested at day 0 and day 24 for flow cytometry analysis. a Histogram plots indicate cellular staining for pluripotent stem cell marker, OCT3, and neural marker, PAX6. b Bar graphs indicate mean fluorescence intensity (MFI) for OCT3 and PAX6. (n = 3 experiments with paired WT and SNV clones. *p ≤ 0.05, **p ≤ 0.01, Kruskal–Wallis, Two stage linear set-up of Benjamini, Krieger and Yekutieli). c NPC light microscopy images, black bar indicates 100 micron. d Venn diagram indicates number of DEGs for EHMT1_WT and EHMT1_SNV during iPSC neural differentiation

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Stem Cell Research & Therapy

ISSN: 1757-6512