Fig. 4
From: Epimorphic regeneration of the mouse digit tip is finite
Repeated amputation inhibits blastema cell expansion and differentiation. A–F Representative images of 5th Amp (upper panel) and 1st Amp blastemas (lower panel) stained for osteoblasts (osterix, green) and proliferating cells (EdU, magenta) from 7 to 14 DPA. Sections were counterstained with DAPI (blue). G, H Immunohistochemical quantification of osteoblasts (Osx+) and proliferating cells (EdU+) normalized over DAPI in regenerating digits after the 5th amputation (n = 5 digits) and 1st amputation (n = 8–10 digits). Scale bar indicates 50 µm. Data are presented as mean ± SD and differences were determined using an unpaired t test. * = p < 0.05; ** = p < 0.01; *** = p < 0.001; **** = p < 0.0001