Fig. 3
From: A dual role of HIF1α in regulating osteogenesis–angiogenesis coupling
2-ME2 alleviated the bone-vessel metabolism of aged mice, but impaired that of young mice. A, B Western blot analysis for HIF1α expression in femurs and tibias. C Representative 3D-reconstructed images by micro-CT, morphological observation by HE staining and immunostaining analysis of VEGF in femurs and tibias. White bar = 500 μM; Black bar = 100 μM; Red bar = 25 μM. D–G BV/TV, Tb.N, Tb,Th, Tb.Sp among groups. H Relative MOD of VEGF by immunostaining. *p < 0.05 versus Young; #p < 0.05 versus Aged