Fig. 4
From: A dual role of HIF1α in regulating osteogenesis–angiogenesis coupling
ABMSCs showed degenerative properties and upregulated HIF1α. A SA-β-gal staining in YBMSCs and ABMSCs. Bar = 100 μM. B, C ALP staining after 7-d osteogenic induction and ARS staining after 21-d osteogenic induction. Bar = 200 μM. D Immunostaining of HIF1α in YBMSCs and ABMSCs. Bar = 50 μM. E Relative gene expression of YBMSCs and ABMSCs after 7-d osteogenic induction. F–H Relative protein expression of YBMSCs and ABMSCs after 7-d and 14-d osteogenic induction, respectively. *p < 0.05 versus YBMSC