Fig. 5

FK866 suppresses mitochondria fusion and OXPHOS. A, C Seahorse mito-stress test for OCR in undifferentiated ST2 cells (A) or osteogenic committed ST2 cells (C) at day 7. The right panels: quantification of OCR parameters. B, D Seahorse mito-stress test for ECAR in undifferentiated ST2 cells (B) or osteogenic committed ST2 cells (D) at day 7. E Representative images of JC-1 staining in ST2 cells. The right panel: quantification of red/green fluorescence intensity ratio in ST2 cells. Scale bar = 100 μm. F Representative transmission electron microscope images of mitochondria in osteogenic committed ST2 cells at day 7. Scale bar in the right panel: 2.0 μm, scale bar in the left panel: 500 nm. G Representative image of western blot analysis with the osteogenic committed ST2 cells at day 7. For (A, C), statistical significance was calculated with Student’s t test. For (E), statistical significance was calculated with two-way ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001. Data are represented as mean ± SD