Table 1 Nucleotide sequence of PCR primers (Homo sapiens)
From: Attenuates of NAD+ impair BMSC osteogenesis and fracture repair through OXPHOS
Gene | Primer F/R | Sequence 5′–3′ |
|---|---|---|
RUNX2 | F | GACTGTGGTTACCGTCATGGC |
RUNX2 | R | ACTTGGTTTTTCATAACAGCGGA |
ALPL | F | GACCTCCTCGGAAGACACTC |
ALPL | R | TGAAGGGCTTCTTGTCTGTG |
SP7 | F | TCTCCATCTGCCTGACTCCT |
SP7 | R | AGCGTATGGCTTCTTTGTGC |
COL1α1 | F | TCTAGACATGTTCAGCTTTGTGGAC |
COL1α1 | R | TCTGTACGCAGGTGATTGGTG |
BGLAP | F | TCACACTCCTCGCCCTATTG |
BGLAP | R | GGGTCTCTTCACTACCTCGC |
SPP1 | F | AGCTTTACAACAAATACCCAGATGC |
SPP1 | R | GGACTTACTTGGAAGGGTCTGTG |
CEBPα | F | CCAGAAAGCTAGGTCGTGGG |
CEBPα | R | TCCTAGGCAATGCTGAAGGC |
PPARγ | F | GGGATCAGCTCCGTGGATCT |
PPARγ | R | TGCACTTTGGTACTCTTGAAGTT |
NAMPT | F | CTTCGGTTCTGGTGGAGGTT |
NAMPT | R | ATCGGCCCTTTTTGGACCTT |
β-actin | F | TCACTATTGGCAACGAGCG |
β-actin | R | AGGTCTTTACGGATGTCAACG |