Fig. 3

MSCs are resistant to APAP injury and protect L-O2 cells from APAP injury. a APAP dose-dependently reduced the viability of L-O2 cells, as determined by MTT analysis, while the reduced viability was not as obvious in MSCs as in L-O2 cells. b Annexin V and 7-AAD staining and flow cytometry analysis showed that APAP did not reduce the number of surviving MSCs but significantly reduced the number of surviving L-O2 cells. c Schematic representation of the co-culture system of L-O2 cells and MSCs in the presence of APAP. d Annexin V and 7-AAD staining and flow cytometry analysis showed that when co-cultured with MSCs (1 × 10⁶), the number of surviving L-O2 cells dose-dependently increased in the presence of APAP (20 mM)