Fig. 4
MSCs dose-dependently protect L-O2 cells from APAP-induced necrosis. a Giemsa staining of the surviving L-O2 cells after treatment with NAC (6 mM) or co-culture with different numbers of MSCs (5 × 105, 1 × 106, 1.5 × 106, 2 × 106 cells) in the presence of APAP (20 mM). b Annexin V and 7-AAD staining and flow cytometry analysis showed that MSCs dose-dependently increased the proportion of surviving L-O2 cells in the presence of APAP (20 mM), and the survival rate of L-O2 cells co-cultured with 2 × 106 MSCs was similar to that of the cells treated with NAC (6 mM)