Fig. 1

Characterization of sEV-AT and ATE. A Illustration of the difference on the preparation of sEV-AT and ATE. B Proteins of sEV-AT and ATE extracted from 1 g adipose tissue were quantified with BCA. The data were analyzed with unpaired two-tailed Student’s t test, ***p < 0.001. C Particles of sEV-AT and ATE extracted from 1 g adipose tissue were quantified with NTA. The data were analyzed with unpaired two-tailed Student’s t test. D Relative particles number and size distribution in 1 μg protein of sEV-AT and ATE. E Particle size distribution of sEV-AT and ATE tested with DLS, dashed lines illustrated the standard error of mean of three measurements. F Total proteins in sEV-AT and ATE were compared by SDS-PAGE and stained with Coomassie brilliant blue. G Proteins enriched in sEV-AT (CD9), ATE (aP2) and both(CAV-1) were validated with western blot