Fig. 2

SHED-Exos inhibit growth and induce apoptosis of endothelial cells. A Viability of endothelial cells analyzed using the CCK-8 assay at different time points. B Immunofluorescence staining of Ki67 to test the proliferation viability of endothelial cells. Scale bars = 500 μm. C Quantification of Ki67-positive cell percentage (%) in B. D HUVECs were incubated with SHED-Exos or PBS (control) for 48 h and analyzed for apoptosis by FACS. E Quantification of apoptotic cell percentage (%) in D. F Western blotting analysis of total and cleaved proteins of PARP, Caspase 9 and Caspase 3 in endothelial cells incubated with exosomes or PBS (control). GAPDH is a housekeeping gene used as a loading control. G Quantification of the relative levels of c-PARP, c-Caspase 9 and c-Caspase 3 in F, as relative change to the respective control cells, after each cleaved protein was normalized to the corresponding total protein band. All experiments were performed three times, and error bars represent means ± SD; P values are indicated with “*”, * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001 when comparing SHED-Exos-treated cells with the control group in C, E and G by Student’s t test