Fig. 5

SHED-Exos suppress micro-vascular formation in vivo. A CAM assays evaluating the anti-angiogenesis ability of SHED-Exos; arrows indicate the injection area. B Quantification of the number of micro-vessels in A. C Schematic diagram of the experimental plan. D Representative images of OSSC tumors harvested from mice three days after the last intra-tumoral injection of SHED-Exos or PBS (control). E Tumor volumes at different time points. F Representative images of micro-vessels around the base of the harvested tumors. G Quantification of the number of vessels in D. H Immunohistochemical staining for CD31 after treatment with SHED-Exos or PBS (scale bars = 200 μm). Images on the right show higher magnifications of areas inside the square boxes on the left (scale bars = 50 μm). I Quantification of the number of CD31 positive cells in the magnified images shown in H. Means and standard deviations are shown in each group in B, E, G and I. *P < 0.05, **P < 0.01, ***P < 0.001