Fig. 6

SHED-Exos transport miR-100-5p and miR-1246 to target VEGFA that inhibits endothelial cell tube formation. A The top 15 highly expressed miRNAs according to miRNA sequencing. B qRT-PCR analysis validating the relative ratio of the expression levels of miR-100-5p and miR-1246 in SHED-Exos and in SHED cells. C left, Matrigel tube formation assay of mimics, inhibitor and NC of miR-100-5p and the tube length and branches number in the network structures of endothelial cells; right, quantification of results shown on the left. D left, Matrigel tube formation assay of mimics, inhibitor and NC of miR-1246 and the tube length and branches number in the network structures of endothelial cells; right, quantification of results shown on the left. E The expression of VEGFA was validated in groups of mimics, mimics NC, inhibitor and inhibitor NC of miR-100-5p by qRT-PCR and Western blot. F The expression of VEGFA was validated in groups of mimics, mimics NC, inhibitor and inhibitor NC of miR-1246 by qRT-PCR and Western blot. G The mTOR and HIF-1a expression levels were analyzed by qRT-PCR in endothelial cells transfected with miR-100-5p mimics and mimics NC. H The target relationship between miR-100-5p and mTOR was verified using a dual-luciferase reporter assay in 293 T cells. I The ACE expression level was analyzed by qRT-PCR in endothelial cells transfected with miR-1246 mimics and mimics NC. All experiments were performed three times, and error bars represent means ± SD; P values are indicated with “*”, * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001