Fig. 7

YY1/SNHG5/miR-212-3p/GDF5/Smad axis is involved in the osteogenesis of hBMSCs. a–d Protein expression of GDF5 and β-actin in groups with YY1 or SNHG5 downregulation or upregulation (n = 3). Histograms show the quantification of band intensities. β-actin was used for normalization. e Relative mRNA expression of GDF5 measured via qRT-PCR in the si-SNHG5-1, si-SNHG5-1 + miR-212-3p-mimic and si-SNHG5-1 + miR-212-3p-inhibitor transfected hBMSCs (n = 3). GAPDH was used for normalization. f Protein expression of GDF5 measured via western blotting in the si-SNHG5-1, si-SNHG5-1 + miR-212-3p-mimic and si-SNHG5-1 + miR-212-3p-inhibitor transfected hBMSCs (n = 3). Histograms show the quantification of band intensities. β-actin was used for normalization. g The top 30 GO term enrichments. h Protein expression of p-Smad1/5/8, smad1/5/8, and β-actin in groups with SNHG5 downregulation or upregulation (n = 3). (*p < 0.05; **p < 0.01). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GDF5, growth differentiation factor 5; GO, Gene ontology; hBMSCs, human bone marrow mesenchymal stem cells; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; SNHG5, small nucleolar RNA host gene 5; YY1, Yin Yang 1