Fig. 3

Neonatal exposure to DES resulted in bigger size testes. Bigger testis showed completely disrupted histo-architecture (a). Seminiferous tubules were significantly reduced without defined membrane and germ cells were depleted (d, e). Sertoli cells were also not observed (d–k). Massive inflammation was observed in testes (i). Degeneration of germ cells was observed. GCNIS like stem cells (arrow) were clearly observed restricted to small seminiferous tubule (d, e, f, g). Small, spherical ‘putative stem cells’ were present among the inflammatory cells (h, i, l). Multinuclear giant cells were observed (b, c). Scale: (a:100  µm, k-l: 20 µm)