Fig. 4

Safety and immunogenecity of ^AlloHSC-iNKT cells. a–b Studying the graft-verus-host (GvH) response of ^AlloHSC-iNKT cells using an in vitro mixed lymphocyte reaction (MLR) assay. PBMC-Tc cells were included as a responder cell control. a Experimental design. PBMCs from 5 different healthy donors were used as stimulator cells. b ELISA analyses of IFN-γ production at day 4 (n = 3). N, no stimulator cells. c–d Studying the GvH response of ^AlloHSC-iNKT cells using NSG mouse model. PBMC-Tc were included as a control. A Experimental design. B Kaplan–Meier survival curves of experimental mice over time (n = 6). e–g Studying T cell-mediated alloreaction against ^AlloHSC-iNKT cells using an in vitro MLR assay. Irradiated ^AlloHSC-iNKT cells (as stimulators) were co-cultured with donor-mismatched PBMC cells (as responders). Irradiated PBMC-Tc cells were included as a stimulator cell control. e Experimental design. PBMCs from 3 different healthy donors were used as responders. f ELISA analyses of IFN-γ production at day 4 (n = 3). g FACS analyses of HLA-I and HLA-II expression on the indicated stimulator cells (n = 5). h–j Studying HLA-I and HLA-II expression on ^AlloHSC-iNKT cells under SARS-CoV-2 infection. ^AlloHSC-iNKT cells were co-cultured with SARS-CoV-2 infected target cells. PBMC-Tc cells were included as a control. h Experimental design. i FACS analyses of HLA-I and HLA-II expression on the indicated stimulator cells. j Quantification of i (n = 5). Representative of 3 experiments. Data are presented as the mean ± SEM. ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, by Student's t test (j and g), by 1-way ANOVA (b and f), or by log rank (Mantel–Cox) test adjusted for multiple comparisons (d)