Fig. 1

Validation of PL10% + GH as an ideal SFM for GSC proliferation. A Microscopic views at 24 h and 72 h of GSCs (n = 4) cultured in PL 1%, 2.5%, 5% and 10% and compared to FCS10% and DEMEM F12 SFM. B and C MTT growth assay at 24 h and 72 h in FCS10%; PL 1%, 2.5%, 5%, 10% and DMEM F12. PL10% medium yielded the highest normalized proliferation rate among SFM for GSCs. D Microscopic views at 24 h and 72 h of GSCs (n = 4) cultured in PL10% and different human supplements: GH, testosterone, EGF, TGFβ1 and bFGF and compared to FCS10% medium. E and F MTT growth assay at 24 h and 72 h in PL10% and different human supplements: GH, testosterone, EGF, TGFβ1 and bFGF and compared to FCS10% medium. PL10% + GH medium yielded the highest rate of GSC proliferation among SFM when compared to PL10% alone. G Cell cycle analysis with CFSE at 24 h of GSC culture in PL 10% and the following single supplements: GH, testosterone, EGF, TGFβ1 and bFGF and compared to FCS10%. Both GH and TGFβ1 increased the proportion of GSCs in S-phase. Scale bars: A 400 µM, D 400 µM