Fig. 4

Immunomodulatory properties of L-GSCs. A Microscopic views of PBMCs after 72 h of culture under two different conditions: non-activated (NA) and activated (A), in the presence or absence of GSCs. PBMCs proliferated around Dyna-beads coupled to anti-CD3/CD28 (A condition), Scale bars: 400 µM. B FACS analysis of Cell Proliferation Dye (CPD) after 72 h of PBMC culture in the NA and A conditions, in the presence or the absence of GSCs. Cells proliferated in the A condition for 3 to 4 generations. C Flow cytometric analysis after 72 h of co-culture revealed that CD4+/CD3+/CD25+/FoxP3+ regulatory T cells were present in the A condition for both S-GSCs and L-GSCs