Table 1 Primers sequences (target genes and housekeeping genes)
Primer | Forward primers (5′–3′) | Reverse primers (5′–3′) |
|---|---|---|
ALP | CGT-GGC-TAA-GAA-TGT-CAT-CAT-GTT | GAT-TTC-CCA-GCG-TCC-TTG-GC |
DSPP | CAA-CCA-TAG-AGA-AAG-CAA-ACG-CG | TTT-CTG-TTG-CCA-CTG-CTG-GGA-C |
GAPDH | GAC-CCC-TTC-ATT-GAC-CTC-AAC-TAC | AAG-TTG-TCA-TGG-ATG-ACC-TTG-GCC |
GH-R1 | GAT-CAG-AGG-CGA-AGC-TCG-GA | AGC-ATC-ACT-TGA-TCC-TGC-CA |
GH-RU | CCA-TTG-CCC-TCA-ACT-GGA-CTT | AAT-ATC-TGC-ATT-GCG-TGG-TGC |
LPL | CAG-GAT-GTG-GCC-CGG-TTT-AT | CAG-GAT-GTG-GCC-CGG-TTT-AT |
OCN | CTT-GGT-GCA-CAC-CTA-GCA-GA | ACC-TTA-TTG-CCC-TCC-TGC-TT |
OPN | AGC-CAG-GAC-TCC-ATT-GAC-TCG-AAC | GTT-TCA-GCA-CTC-TGG-TCA-TCC-AGC |
PPAR | GCG-ATT-CCT-TCA-CTG-ATA-C | CTT-CCA-TTA-CGG-AGA-GAT-CC |
SDHA | AGC AAG CTC TAT GGA GAC CT | TAA TCG TAC TCA TCA ATC CG |
TGFß-R1 | TGG-TAC-CCA-AGG-AAA-GCC-AG | CCA-CTC-TGT-GGT-TTG-GAG-CA |
UBC | GTG GCA CAG CTA GTT CCG T | CTT CAC GAA GAT CTG CAT TGT CA |