Fig. 3

LPS alters the regulation of AT2 lineage by prkg2 genes. A DIC images on day 8. Scale bar, 1 mm. B Colony formation on day 8 (n = 6 wells, two wells per mouse, three mice per group). C Confocal images of AT2 organoids. Scale bar, 50 μm. D Epithelial cell count (n = 4 organoids, 1–2 organoids per well, one well per mouse, three mice per group). E AT1:AT2 cell ratio (n = 4 organoids, 1–2 organoids per well, one well per mouse, three mice per group). F Representative images of EdU-stained organoids. Scale bar, 50 μm. G Percentage of EdU⁺ cells (n = 10 organoids, 1–2 organoids per well, two wells per mouse, three mice per group). Data are represented as ± SEM, **p < 0.05, **p < 0.01, and ***p < 0.001 versus controls by two-tailed Student's t-test