Table 1 Oligonucleotides and primer sequences
Oligonucleotide name | Sequence |
|---|---|
Evx1 guide 21 F | ACACCTCGCGGGCTCAGGCAACCGTG |
Evx1 guide 21 R | AAAACACGGTTGCCTGAGCCCGCGAG |
Evx1 guide 28 F | ACACCAAGTCGGAGGGCCGGCTCGCG |
Evx1 guide 28 R | AAAACGCGAGCCGGCCCTCCGACTTG |
5′ Evx1 homology arm F | GGGGACAAGTTTGTACAAAAAAGCAGGCTCTGTAGCTATGGATTCT |
5′ Evx1 homology arm R | CTCGGTCATATTGGCAAATTAGAGACCCTC |
PAC cassette F | GAGGGTCTCTAATTTGCCAATATGACCGAG |
PAC cassette R | GTCTGAGCTACTAGTCTAGAACTAGTGGATCC |
3′ Evx1 homology arm F | GGATCCACTAGTTCTAGACTAGTAGCTCAGAC |
3′ Evx1 homology arm R | GGGGACCACTTTGTACAAGAAAGCTGGGTTCGGTCCTTTATATTC |
5′ genomic Evx1 F | GGAACGGGTACTTTAGGCTC |
5′ PAC insertion R | TCGTAGAAGGGGAGGTTGC |
3′ PAC/Neo insertion F | TCTGGATTCATCGACTGTGG |
3′ genomic Evx1 R | TTGGGACCATTTCCGACCTG |