Fig. 5

IFITM3 increases the survival and maintains the proliferation of mNRPCs mainly through the CMA signaling pathway. A The expression of mTOR pathway-related proteins in cells after IFITM3 knockdown. B The expression of ERK1/2 was significantly decreased in IFITM3-knockdown cells. C The expression of BCL2 and P53 in IFITM3-knockdown cells. D The expression of MA pathway-related proteins in cells after IFITM3 knockdown. E CMA was activated, accompanied by increased expression of LAMP2A and HSC70 in cells in which the IFITM3 gene was knocked down. The results are representative of at least three independent experiments, and representative blots are shown. F Bar graph showing the relative protein expression levels from the western blot. G Results of IHC analysis of LC3A/B and fluorescent images showing mCherry-GFP-LC3 expression after IFITM3 knockdown used to assay autophagic flux in the cells. Magnification: × 400; scale bar: 50 μm