Fig. 3

Overexpression of Hira or Ubn2 rescues the expression of retrotransposons. a The expression levels of Hira after transfected with Hira shRNA in control OE ESCs and Hira OE ESCs, as measured by RT-qPCR and normalized to Gapdh level; ***p < 0.001 in Student’s t test. b The expression levels of Ubn2 after transfected with Ubn2 shRNA in control OE ESCs and Ubn2 OE ESCs, as measured by RT-qPCR and normalized to Gapdh level; ***p < 0.001 in Student’s t test. c The expression levels of Ubn1 after transfected with Ubn1 shRNA in control OE ESCs and Ubn1 OE ESCs, as measured by RT-qPCR and normalized to Gapdh level; ***p < 0.001 in Student’s t test. d–f Immunoblot analysis of the expression of Hira (d), Ubn2 (e), or Ubn1 (f) after overexpression of Hira, Ubn2, or Ubn1. Actin was included as a loading control. g–i qPCR analysis of ERVs expression in Hira (g), Ubn2 (h) and Ubn1 (i) rescue ESCs lines. The results were normalized to Gapdh. Data are represented as mean ± s.e.m. (n = 3 independent experiments) for the above qPCR results. ns: non-significant, **p < 0.01; ***p < 0.001 in Student’s t test