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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Enhancement of pacing function by HCN4 overexpression in human pluripotent stem cell-derived cardiomyocytes

Fig. 2

Generation of HCN4-overexpressing human induced pluripotent stem cells (iPSCs). A Confirmation of transgene integration in the AAVS1 locus by PCR. The 2023-bp product means homologous recombination (HR) and the 2575-bp product means random integration. Clone #5 of HCN4/EGFP iPSCs and clone #11 of EGFP iPSCs were mainly used in this study. B Undifferentiated markers expressed in HCN4-overexpressing and non-overexpressing iPSCs. C EGFP and HCN4 expression in HCN4-overexpressing and non-overexpressing iPSCs. Scale bar is 100 µm

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Stem Cell Research & Therapy

ISSN: 1757-6512