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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Exosomes from adipose-derived stem cells regulate M1/M2 macrophage phenotypic polarization to promote bone healing via miR-451a/MIF

Fig. 5

ADSC-Exos promote the polarization of LPS/IFN-γ-treated M1 macrophages towards the M2 phenotype. U937-derived M1 macrophages were pretreated with ADSC-CM and ADSC-Exos followed by stimulation with LPS/IFN-γ. A The protein levels of the M1 macrophage markers iNOS and CD86 and the M2 macrophage marker CD206 were analysed by western blotting. B M1 macrophage markers and M2 macrophage markers were detected at the mRNA level by RT-qPCR. ADSC-Exos (C) and released from GNPs’ADSC-Exos (D) were labelled with PKH67 (green), cocultured with macrophages for 0.5 h and 1 h, and fluorescence signals were examined by fluorescence microscopy. Data are representative of at least two independent experiments. The significance levels are indicated: ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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Stem Cell Research & Therapy

ISSN: 1757-6512