Fig. 4

EVs uptake by HDFs and proliferation assay. a EVs flow cytometry uptake: (I) Gating of the cell populations and percentage of positive events for the background control (Ctrl DIO), and the EV preparations of 10 K and 100 K for HF and AT cells. (II) The upper table shows the percentage of fluorescent cells after adding different preparations of EVs. Bottom table shows the number of vesicles per well added for each preparation. (III) The graphs present the percentage of positive events according to the flow cytometry study corrected by the number of EVs for each type of EVs. *p < 0.05, N.S. Non-significance. b Representative fluorescence micrographs of the capture of EVs and region of interest (ROI) for each preparation. The figure at the bottom show HDFs treated with the negative control corresponding to the carry-over of fluorescence of cell culture media never conditioned with HDFs, and untreated HDFs. c HDFs proliferation assay by CCK8 (n = 3). N.S. Non-significant, *p < 0.05; **p < 0.005; ***p < 0.001 against the control group; ^#p < 0.05 against the positive control group; ^&&&p < 0.001 between groups