Fig. 4

Small-molecule cocktail VDF directed hepatic specification. A Schematic of the strategy to identify chemical culture cocktails for HB specification. B RT-PCR analyzed AFP and HNF4α expression during hepatic specification. Data are presented as mean ± SEM, n = 3. C Immunostaining analyses of AFP and HNF4α expansion after 5 days of BF induction. Scale bars 100 μm. D RT-PCR results show hepatic genes expression in differentiated cells induced by different small molecule cocktails. Data are presented as mean ± SEM, n = 3. E Immunostaining analyses of AFP and HNF4α expression after different small molecule cocktails induced. F The efficiency of AFP and HNF4α expression after different chemical cocktails treatment, determined by counting positive cells. Efficiencies are presented as the percentage of positive cells plus or minus the SD of all fields counted